In this video, we look at how organelles are separated by cell fractionation. First we look at the roles of a homogeniser and buffer. We then look at the relative sizes of the different organelles. Finally, we explore how the organelles are fractionated by ultracentrifugation.
Image credits:
Pancreatic acinar cells
https://commons.wikimedia.org/wiki/File:Pancreatic_acinar_cells_-_TEM.jpg
Louisa Howard, Public domain, via Wikimedia Commons
Heart dissected
Samuel Levy, CC BY 3.0 https://creativecommons.org/licenses/by/3.0, via Wikimedia Commons
Homogeniser
https://commons.wikimedia.org/wiki/File:Tissue_glass_teflon_Dounce_homogenizer-02.jpg
Lilly_M, CC BY-SA 3.0 https://creativecommons.org/licenses/by-sa/3.0, via Wikimedia Commons
Centrifuge
https://commons.wikimedia.org/wiki/File:Multipurpose_centrifuge.JPG
Retama, CC BY-SA 4.0 https://creativecommons.org/licenses/by-sa/4.0, via Wikimedia Commons
